C8 Column HPLC, USHB C8 Reversed Phase HPLC Column

C8 Column HPLC, USHB C8 Reversed Phase HPLC Column

C8 Column HPLC, USHB C8 Reversed Phase HPLC Column

Short Description:

C18 columns are the most commonly used columns in reversed-phase analytical methods. The high-purity silica matrix is bonded with a universal C18 functional group, and multiple blocking makes the column less adsorbed to ionized compounds. The optimized surface design results in a column with a higher specific surface area, higher carbon content, stronger hydrophobic retention, and higher column efficiency, thus ensuring a better separation.

The synthesis of silica gel packing, chemical treatment of silica gel surface, and loading of packing are all tested and controlled by strict quality standards to ensure good batch reproducibility of the column. It is ideal for method development and complex component analysis.

  • Remarks: Custom designs and fittings available
  • Product Detail


    Product Tags

     - Suitable for separating most hydrophobic compounds

     When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to a C8 column. The C8 can be the ideal phase for non-polar compounds that are retained too strongly by a traditional C18 column.

    Particle Size

    3um, 5um, 7um, 9um, 10um …

    Pore Size

    70 Å, 120 Å, 150 Å, 200 Å …

    Carbon Content









    1. What is the difference between C8 and C18 columns used in an HPLC system?

    - Octadecylsilane (C18) has 18 carbon atoms. On the other hand, Octylsilane(C8) has only 8 carbon atoms on the column parking bonded to silica (Si).C18 will tend to retain more than C8.In that, if a similar compound was eluted on the two columns, it will elute faster on C8 and slower on C18. The C8 column is more polar than the C18 column because it has 10 fewer C chains, so the retention of the same substance on C8 is weaker than that on C18. So if your sample is very polar, you can consider using a C8 column. For example, some protein analyses will use C8 or C4 column.


    2. Can C8 and C18 columns be used interchangeably?

    - These two chromatographic columns are commonly used in reverse chromatography. The C8 column and the C18 column have different polarities and separate polar substances. Whether they can be used interchangeably to analyze samples depends on your analysis. What is the sample? For example, to analyze melamine, these two columns can be used. These two chromatographic columns are based on silica gel. In silica gel, there are groups that are not bonded. One bond

    The one bonded is carbon octadecyl, and the one bonded is carbon octadecyl.

    laboratory illustration

    uHPLCs special services:
    1. All columns will be tested before delivery and a column efficiency report will be provided.
    2. Column loading service is available.23030101 https://www.uhplcslab.com/

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